割 研 332 2021-2026 2021 撑 覆 ⾝ 95 研 BCC research 2026 (CAGR) 28.4% 筛 封 割 撑练 割 务 模 - - 封 DBTL 的 封 1 展 ................................. 3 1.1 .......................................................................................................................... 3 1.1.1 ...................................................................................................................... 3 1.1.2 DBTL ................................................................................................................................ 4 1.1.3 ................................................................................................................................... 5 1.1.4 ............................................................................................................ 8 1.2 ........................................................................ 9 1.2.1 筛 ........................................................................................................................................ 9 1.2.2 ......................................................................................................................................10 1.3 ..........................................................................................................13 1.4 Ginkgo Bioworks ............................................................15 2 展 2.1 2.2 2.2.1 2.2.2 SDLs 2.3 2.3.1 2.3.2 2.4 2.4.1 2.4.2 2.4.3 2.4.4 iBioFoundry 3 展 3.1 3.2 3.3 4 .............................................. 18 - DBTL ................................................................18 ..................19 ........................20 ................................................................................26 ....................................27 Biofoundry TALENs ........................29 BioAutomata .............................................................................31 ................................33 ......................................................................................................34 ...............................................................................................................35 ..........................................................................................................37 务 ........................................................................................38 ........................................... 40 .............................................................................................................................40 SDLs .............................................................................................41 SDLs ...........................................................................................................43 ....................................................................... 44 2 1 展 1.1 1.1.1 Synthetic Biology 去 封 导 DNA 研 封 去 勃 1953 2003 National Institutes of Health, NIH National Genome Research Institute 覆 撑 撑练 DNA 封 确 20 21 告 去 3 1.1.2 DBTL 撑 练 撑练 - - DBTL DBTL 撑 1. 1. - - - 覆 Design DBTL 去 务 务 DNA 去 2. Build DNA DNA 封 务 割 的 割 研 DNA DNA 封 BioBrick BglBricks In-Fusion Gateway Golden Gate Start-Stop DNA (Oligonucleotides linkers mediated DNA assembly OLMA) (Zinc finger nucleases ZFN) 去 TALEN 封 去 CRISPR-Cas 4 去 CRISPR-Cas CRISPR-Cas9 CRISPR-Cas12a (Cpf1) 割 务 务 3. Test 盟 RBS 去 去 封 DBTL DBTL 研 25 务 ( 100 4. ) 300 600 > 90% /d /d 撑练 Learning DBTL 务 -RNA- 割 覆 研 -去 - 去 研 研 告 研 研 去 研 研 DBTL 割 1.1.3 Ÿ 够 / / Ÿ 5 Ÿ 导 撑练 去 DBTL 1. DNA DNA DNA DNA 去 DNA 封 1) 务 2) 3) Sanger DNA 2. 去 去 DNA DNA DNA 研 21 封 2. DNA 务 DNA DNA DNA 3. 3 ZFN 6 TALEN DNA CRISPR/Cas 去 去 CRISPR/Cas9 1) 2) 3) ZFN TALEN CRISPR/Cas9 ZFN CRISPR-Cas TALEN Cas CRISPR 务 3. CRISPR CRISPR-Cas 封 Strategies to overcome the main challenges of the use of CRISPR/Cas9 as a replacement for cancer therapy 4. DNA DNA DNA DNA DNA DNA DNA ligase chain reaction, LCR assembly, PCA 务 DNA polymerase cycling DNA 群 封 7 1.1.4 告 封 synthetic biology 2000 Stephane Leduc 封 1910 告 Nature 封 割 去 DNA 去 封 封 割 10 Ÿ 10 2003 去 务 去 务 Ÿ 2004 - 2007 Ÿ 的 告 2008 - 2013 去 Ÿ Amyris 2014 研 4. 封 去 8 - - 务 告封 封 封 割 研 资 割 封 1.2 1.2.1 筛 裂 封 筛 封 务 封 筛 / 筛 封 筛 封 务 封 够 封 筛 封 封 2016 筛 封 封 2012 够 封 3 封 去 2019 去 封 筛 L- 6-APA 筛 封 973 863 够 2022 5 封 封 够 封 封 9 封 封 筛 5. 封 1.2.2 2021 Zymergen Ginkgo IPO 尽 2021 研 2009-